Propidium iodide

Propidium Iodide cannot cross. PI binds to DNA by intercalating between the bases with little or no sequence preference. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.

Analysis of apoptosis by propidium iodide staining and flow cytometry. Find product specific information including CAS, MSDS, protocols and references.

Riccardi C(1), Nicoletti I. For nucleic acid staining in flow and immunofluorescence. Membrane impermeable DNA intercalator. Find MSDS or SDS, a COA, data sheets and more information. Useful for flow cytometry for staining apoptotic cells and nuclei. Because it is membrane impermeable the state of the cell determines the ability of propidium iodide from staining viable and apoptotic cells.

Once bound to nucleic acids, the nuclei appear red in color as observed by fluorescence microscopy with a rhodamine filter. PI is useful for multi-color fluorescent staining analysis.

The bright red staining of nuclei . The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging. Assess cell health with our ready-to-use propidium iodide solution. Bypass weighing, pipetting, and dilution steps in cell sorting and flow cytometry studies.

Used as counter stain of nuclei in Annexin staining (detection of apoptotic cells). For the study of micronuclear morphology of protozoa (3). It is widely used as a counterstain to differentiate and exclude non-viable cells in flow cytometric analyses, . PI is not membrane permeable and therefore requires the com. Dead cells will take up PI and fluoresce brightly in FLoff of the blue laser on a standard flow cytometer.

As a nuclear counterstain, propidium iodide stains the nucleus light to dark red in color. The fluorescent red color provides excellent contrast to green or blue fluorochromes such as FITC, Cyor AMCA. Regulatory Status: RUO . Learn more about propidium – iodide. We enable science by offering product choice, services, process excellence and our people make it happen.

PI rapidly enters cells with compromised membranes and intercalates between base pairs allowing exclusion of non-viable cells from analysis of flow cytometr. Quantity in Basket: None. Cell membrane integrity excludes propidium iodide from staining viable and apoptotic cells.